Amperometric biosensor for NADH and ethanol based on electroreduced graphene oxide-polythionine nanocomposite film

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• 作者：Zou LiAuthor Vitae ; Yi HuangAuthor Vitae ; Li ChenAuthor Vitae ; Xiaoli QinAuthor Vitae ; Zhao HuangAuthor Vitae ; Yaping ZhouAuthor Vitae ; Yue MengAuthor Vitae ; Jing LiAuthor Vitae ; Siyu HuangAuthor Vitae ; Yan LiuAuthor Vitae ; Wen WangAuthor Vitae ; Qingji Xie ; ^{xieqj@hunnu.edu.cn}Author Vitae ; Shouzhuo YaoAuthor Vitae
• 关键词：Graphene&ndash ; polythionine nanocomposite film ; Alcohol dehydrogenase ; NADH ; Amperometric sensing ; Electrochemistry quartz crystal microbalance
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：181
• 期：Complete
• 页码：280-287
• 全文大小：1395 K

文摘

A nanocomposite film is prepared by cyclic voltammetric deposition of electroreduced graphene oxide (ERGO) and polythionine (PTH) on a glassy carbon (GC) electrode. The electrodeposition of ERGO-PTH film is also investigated via the electrochemical quartz crystal microbalance (EQCM) method for more in-depth understanding of the process. The ERGO-PTH nanocomposite film is applied as transducer for facilitated electrocatalytic oxidation of NADH and for the construction of dehydrogenase-based amperometric biosensor. Under optimum conditions, the amperometric detection of NADH provides a wide linear detection range (0.01-3.9 mM), a high sensitivity (143 ¦ÌA mM^?1 cm^?2) and a low limit of detection (LOD = 0.1 ¦ÌM, S/N = 3). With alcohol dehydrogenase (ADH) as a model, we obtain a linear amperometric response of the ADH-modified ERGO-PTH/GC electrode to ethanol concentration from 0.05 to 1.0 mM, a sensitivity of 2.8 ¦ÌA mM^?1 cm^?2, and a LOD of 0.3 ¦ÌM (S/N = 3).