Evaluation of toxicological biomarkers in secreted proteins of HepG2 cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and their expressions in the plasma of rats and incineration workers
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- 作者：Sohee Pharka ; 1 ; So-Young Parkb ; 1 ; Yoon-Seok Changc ; Seonyoung Choia ; Ji-youn Lima ; Yoonjin Kima ; Jong Bok Seod ; Woon-Won Junge ; Donggeun Sula ; dsul@korea.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：MTT ; 3-(4 ; 5-dimethyl thiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; LC&ndash ; ESI-MS/MS ; liquid chromatography electrospray ionization&ndash ; tandem mass spectrometry ; TOF2 MS ; time-of-flight 2 mass spectrometry ; IEF ; isoelectric focusing ; SDS-PAGE ; sodium dodecyl sulfate-polyacrylamide gel electrophoresis ; 2-DE ; two dimensional gel electrophoresis
- 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：1864
- 期：5
- 页码：584-593
- 全文大小：1776 K
文摘
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The toxicological biomarkers of 2,3,7,8-TCDD in secreted proteins were evaluated using HepG2 cells.
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Eight secreted proteins were confirmed by western blotting.
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PSMB 5 and PRX 1 were validated in rat plasma.
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GLO 1, HGD, PSMB 6 and PRX 1 were found in the plasma of incineration workers.
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These proteins could be used as potential toxicological biomarkers of 2,3,7,8-TCDD.