Poly-pathway model, a novel approach to simulate multiple metabolic states by reaction network-based model - Application to amino acid depletion in CHO cell culture
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- 作者：Erika Hagrota ; Hildur Æ ; sa Oddsdó ; ttirb ; Joan Gonzalez Hostaa ; Elling W. Jacobsenc ; Vé ; ronique Chotteaua ; chotteau@kth.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Modeling ; Metabolic flux analysis ; Elementary flux modes ; Chinese hamster ovary cells ; Amino acid metabolism ; Poly-pathway model
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 June 2016
- 年：2016
- 卷：228
- 期：Complete
- 页码：37-49
- 全文大小：4236 K
文摘
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A method for modeling multiple states in one model, named poly-pathway, is proposed.
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The macroscopic model is based on elementary flux modes and generalized kinetics.
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CHO cells were cultured under varied amino acid availability.
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Distinct metabolic states were obtained.
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A single model simulates observed variations in growth and metabolic rates.