Combination of O3-NaOH-Ultrasound as pretreatment of sugar cane was evaluated.
O3-NaOH-Ultrasound pretreatment promotes high delignification of biomass.
O3-NaOH-Ultrasound pretreatment occurs at ambient temperature and pressure.
Glucose yield of 391 mg/g (95%) was obtained after pretreatment.
Changes in fiber due to the pretreatment have been demonstrated.