Functional variants of 5S rRNA in the ribosomes of common sea urchin Paracentrotus lividus

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• 作者：Eufrosina Dimarco ; Eleonora Cascone ; Daniele Bellavia ; Fabio Caradonna
• 关键词：rRNA ; ribosomal ribonucleic acids ; rDNA ; ribosomal deoxyribonucleic acids ; SSCP ; single-strand conformation polymorphism ; bp ; base pairs ; RT ; reverse transcription ; PCR ; polymerase chain reaction
• 刊名：Gene
• 出版年：2012
• 出版时间：15 October, 2012
• 年：2012
• 卷：508
• 期：1
• 页码：21-25
• 全文大小：517 K

文摘

We have previously reported a molecular and cytogenetic characterization of three different 5S rDNA clusters in the sea urchin Paracentrotus lividus; this study, performed at DNA level only, lends itself as starting point to verify that these clusters could contain transcribed genes, then, to demonstrate the presence of heterogeneity at functional RNA level, also. In the present work we report in P. lividus ribosomes the existence of several transcribed variants of the 5S rRNA and we associate all transcribed variants to the cluster to which belong.

Our finding is the first demonstration of the presence of high heterogeneity in functional 5S rRNA molecules in animal ribosomes, a feature that had been considered a peculiarity of some plants.