The role of Galectin-1 in the interaction between chondrocytes and a lactose-modified chitosan

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• 作者：Marcon ; Patrizia ; Marsich ; Eleonora ; Vetere ; Amedeo ; Mozetic ; Pamela ; Campa ; Cristiana ; Donati ; Ivan ; et. al.
• 关键词：Galectin-1 ; Glycopolymer ; Biomaterial ; Chondrocyte ; Cartilage
• 刊名：Biomaterials
• 出版年：2005
• 出版时间：August, 2005
• 年：2005
• 卷：26
• 期：24
• 页码：4975-4984
• 全文大小：278 K

文摘

Evidences for the involvement of the Galectin-1 in the interaction of pig chondrocytes with a lactose-modified chitosan, namely Chitlac, are reported. The Chitlac glycopolymer has been shown to promote pig chondrocyte aggregation and to induce extracellular matrix production. Highly pure Galectin-1 was obtained from pig spleen by affinity chromatography and its identity was determined by ion spray mass spectrometry analysis of tryptic peptide fragments obtained after in-gel digestion. The complete sequence of pig Galectin-1 CDS was obtained by screening a pig EST database using human Galectin-1 sequence as template. The Galectin-1 cDNA was cloned into a pGEX-4T-1 expression vector and the recombinant protein was purified, characterized and used to produce a rabbit anti-serum. Recombinant Galectin-1 interacts in a dose-dependent manner with Chitlac as determined with ELISA assay. Expression level of galectin-1 gene, quantified by real-time PCR, was significantly higher in chondrocytes cultivated on Chitlac. In the same way, the presence of Chitlac stimulates secretion of Galectin-1 in culture medium that, by immunohistochemical analysis, revealed to be clustered on the surface of Chitlac-induced aggregates. These data indicate the role of Galectin-1 as a bridging agent between Chitlac and chondrocyte aggregates.