Phylogeny of Murray Valley encephalitis virus in Australia and Papua New Guinea

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• 作者：Eleonora Cella¹ ; ² ; Ivan Gabrielli³ ; Gianguglielmo Zehender⁴ ; Marta Giovanetti¹ ; ⁵ ; Alessandra Lo Presti¹ ; Alessia Lai⁴ ; Giordano Dicuonzo⁶ ; Silvia Angeletti⁶ ; Marco Salemi⁷ ; Massimo Ciccozzi¹ ; ⁸ ; ^{ciccozzi@iss.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Murray valley encephalitis virus ; Phylogeny ; Evolution
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：9
• 期：4
• 页码：385-389
• 全文大小：785 K

文摘

To study the genetic diversity of Murray Valley encephalitis virus (MVEV) in Australia and Papua New Guinea.

Methods

MVEV envelope gene sequences were aligned using Clustal X and manual editing was performed with Bioedit. ModelTest v. 3.7 was used to select the simplest evolutionary model that adequately fitted the sequence data. Maximum likelihood analysis was performed using PhyML. The phylogenetic signal of the dataset was investigated by the likelihood mapping analysis. The Bayesian phylogenetic tree was built using BEAST.

Results

The phylogenetic trees showed two main clades. The clade Ⅰ including eight strains isolated from West Australia. The clade Ⅱ was characterized by at least four epidemic entries, three of which localized in Northern West Australia and one in Papua New Guinea. The estimated mean evolutionary rate value of the MVEV envelope gene was 0.407 × 10⁻³ substitution/site/year (95% HPD: 0.623 × 10⁻⁴–0.780 × 10⁻³). Population dynamics defines a relative constant population until the year 2000, when a reduction occurred, probably due to a bottleneck.

Conclusions

This study has been useful in supporting the probable connection between climate changes and viral evolution also by the vector point of view; multidisciplinary monitoring studies are important to prevent new viral epidemics inside and outside new endemic areas.