Towards a multi-site synthetic vaccine to foot-and-mouth disease: addition of discontinuous site peptide mimic increases the neutralization response in immunized animals
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- 作者：Villé ; n ; Judit ; Oliveira ; Eliandre de ; Nú ; ñ ; ez ; José ; Ignacio ; et. al.
- 关键词：Multi-epitope peptide vaccines ; Discontinuous epitopes ; Dendrimeric presentation ; AAA ; amino acid analysis ; Ada ; 2-aminodecanoic acid ; Boc ; tert-butyloxycarbonyl ; BrAc ; bromoacetyl ; BSA ; bovine serum albumin ; CFA ; complete Freund’ ; s adjuvant ; C
- 刊名：Vaccine
- 出版年：2004
- 出版时间：September 9, 2004
- 年：2004
- 卷：22
- 期：27-28
- 页码：3523-3529
- 全文大小：213 K
文摘
Synthetic replicas of both antigenic sites A and D of foot-and-mouth disease virus have been tested as a first step towards a multicomponent peptide vaccine candidate. A first evaluation has been performed by neutralization assays on cells with serum mixtures from guinea pigs immunized independently with site A (A24) and site D (D8) peptides. The addition of site D antibodies to site A antibodies has a synergistic effect on neutralization. In a second group of experiments, guinea pigs have been immunized with a dendrimeric tetravalent (MAP) presentation of site A peptide, alone or in combination with D8, using the same total peptide dose. While the first inoculation gives a preferential response to site A-only antigen, specific response to site D and global neutralization levels significantly increase after reimmunization, reflecting a synergistic effect of site D.