Development of a novel rapid micro-neutralization ELISA for the detection of neutralizing antibodies against Chandipura virus
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- 作者：R.G. Damle ; rgdamle2000@gmail.com ; A.A. Patil ; V.S. Bhide ; S.D. Pawar ; G.N. Sapkal ; V.P. Bondre
- 关键词：CHPV ; India ; MN-CPE ; VT-ELISA ; MN-ELISA ; Nabs
- 刊名：Journal of Virological Methods
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：240
- 期：Complete
- 页码：1-6
- 全文大小：559 K
- 卷排序：240
文摘
Development of MN-ELISA using Indian strain of CHPV to detect Nabs to CHPV. MN-ELISA gives read-out in the form of optical density values and has a shorter TAT. The sensitivity, specificity, positive predictive value and negative predictive value of the new test was 100% when compared with the conventional MN-CPE method. New assay detects very minute difference in Nab titer and also low-titer sero-conversion. MN-ELISA; a better alternative to the conventional MN-CPE in sero-surveillance and in future vaccine studies.