Cysteine cathepsins in human silicotic bronchoalveolar lavage fluids

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• 作者：Cé ; line Perdereau ; Emmanuel Godat ; Marie-Christine Maurel ; Eric Hazouard ; Elisabeth Diot and Gilles Lalmanach
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Basis of Disease
• 出版年：2006
• 出版时间：March 2006
• 年：2006
• 卷：1762
• 期：3
• 页码：351-356
• 全文大小：169 K

文摘

Mature, active cysteine cathepsins (CPs) were identified in human inflammatory bronchoalveolar lavage fluid (BALF) supernatants from patients suffering from silicosis by both western blot and surface plasmon resonance analyses. BALFs are not a reservoir of activatable proforms, since no autocatalytic maturation at acidic pH occurs. Cathepsin H is the most profuse among studied CPs (median value: 36.5 nM), while cathepsins B and L are the two most abundant thiol-dependent endoproteases. The overall concentration of active cathepsins B, H, K, L, and S is 10-fold lower than their concentration in BALF supernatants from patients suffering from inflammatory acute lung injuries (962 ± 347 nM).The cathepsins (approximately 70 nM)/cystatin-like inhibitors (approximately 9 nM) ratio is unbalanced in favor of enzymes (8-fold). This presence of uncontrolled CPs suggests that they may contribute, in addition to matrix metalloproteases, to the lung tissue breakdown/remodeling occurring during silicosis, although their exact contribution to interstitial inflammation remains to be evaluated.