A statistical approach to determining responses to individual peptides from pooled-peptide ELISpot data
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- 作者：Peter Strö ; ma ; Nathalie Stø ; era ; Nicola Borthwickb ; Tao Dongc ; Tomá ; &scaron ; Hankeb ; c ; Marie Reillya ; marie.reilly@ki.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：ELISpot assay ; Peptide pools ; EM-algorithm ; Background adjustment
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：435
- 期：Complete
- 页码：43-49
- 全文大小：535 K
文摘
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ELISpot assays of peptide pools enable the estimation of responses to individual peptides.
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There was good agreement between estimates and confirmatory assays in an application to HIV-1 immunogenicity.
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The estimates can inform substantial reductions in the number confirmatory assays.
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We provide an easy-to-use free online tool for obtaining the individual-peptide estimates.
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The method enables more efficient use of information from pooled ELISpot assay data.