Cloning and sequencing of a novel murine liver carboxylesterase cDNA
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- 作者：Ellinghaus ; Peter ; Seedorf ; Udo ; Assmann ; Gerd
- 关键词：Carboxylesterase ; Differential display PCR (DD-PCR) ; Sterol carrier protein 2 (SCP2) ; Cholestyramine
- 刊名：Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression
- 出版年：1998
- 出版时间：April 29, 1998
- 年：1998
- 卷：1397
- 期：2
- 页码：175-179
- 全文大小：409 K
文摘
Carboxylesterases (EC 3.1.1.1) comprise a group of serine hydrolases with at least 20 genetically distinct loci in mice. Here, we describe differential display PCR-based cloning of a cDNA, encoding a novel murine carboxylesterase termed ES-x, which was expressed predominantly in liver but also in kidney and lung. The cDNA of ES-x spanned a 2249-bp sequence with an open reading frame encoding 565 amino acids, including an N-terminal hydrophobic signal peptide which directs the synthesis into microsomal lumen and a C-terminal HVEL consensus sequence for retaining the protein in the lumen of the endoplasmic reticulum. The predicted amino acid sequence of ES-x exhibited 75 % identity with rat liver pI 6.1 esterase. We further demonstrate that feeding mice with diets containing cholestyramine or sodium cholate increases mRNA-expression of ES-x in liver 2.5- to 3-fold.