Methylation-dependent and independent regulatory regions in the Na,K-ATPase alpha4 (Atp1a4) gene may impact its testis-specific expression
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- 作者：Deepti L. Kumar1 ; Priya L. Kumar1 ; Paul F. James ; jamespf@miamioh.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：bp ; base pair ; CGI ; CpG island ; CMV ; human cytomegalovirus ; Dnmt ; DNA methyltransferase ; DMEM ; Dulbecco's Modified Eagle's media ; DKO ; double knockout ; ES cell ; mouse embryonic stem cell ; FBS ; fetal bovine serum ; HB-EGF-C ; C-terminal fragment of heparin-binding EGF-like growth factor ; HEK 293 ; human embryonic kidney 293 ; IACUC ; University Institutional Animal Care and Use Committee ; IV ; in vitro methylated ; kDa ; kilodalton ; KO ; knockout ; LIF ; leukemia inhibitory factor ; Mα4-CGI ; intragenic CpG i
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：339-352
- 全文大小：2032 K
文摘
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A differentially methylated intragenic CpG island is present in the Atp1a4 gene.
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Maintenance and de novo methylation are involved in regulation of Atp1a4 expression in ES cells.
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Methylation of the Mα4-Promoter regulates its activity.
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Methylation of the intragenic Mα4-CGI alters transcription elongation efficiency.
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The Mα4-CGI displays silencer activity which may serve to fine tune Atp1a4 expression in germ cells.