The relationship between graft incompatibility and phenols in Uapaca kirkiana

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• 作者：Simon A. Mng’ ; omba ; Elsa S. du Toit ; Festus K. Akinnifesi
• 关键词：Callus ; Euphorbiaceae ; Exudates ; Field survival ; Secondary metabolites
• 刊名：Scientia Horticulturae
• 出版年：2008
• 出版时间：23 July 2008
• 年：2008
• 卷：117
• 期：3
• 页码：212-218
• 全文大小：2336 K

文摘

The objective of the study was to determine the relationship between phenols and graft incompatibility in Uapaca kirkiana. Phenol quantification and identification were carried out using Folin-Ciocalteau reagent procedure, fluorescence microscopy and reverse phase high performance liquid chromatograph (RP-HPLC) above, below and at the graft union. Results showed no vascular cambium continuity above the scion/stock unions. Significant differences in total soluble phenols and cell wall bound phenols were obtained. Fluorescence microscope indicated the presence of flavonoids and other polymers above the union. The RP-HPLC identified ferulic acid as a major phenol component found in U. kirkiana plant cells and responsible for wood discolouration. High phenol concentrations were obtained in less compatible combinations than in compatible combinations. High peaks of ρ-coumaric acid were obtained above the union. It is concluded that phenols, especially ρ-coumaric acids and flavonoids caused poor callus formation at the union, and hence implicated in graft incompatibility.