Is the cytoplasmic loop of MerT, the mercuric ion transport protein, involved in mercury transfer to the mercuric reductase?

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• 作者：Rossy ; Emmanuel ; Sé ; nè ; que ; Olivier ; Lascoux ; David ; Lemaire ; David ; Crouzy ; Serge ; et. al.
• 关键词：Mercury transport ; Heavy-metal resistance ; Mass spectrometry ; ¹H NMR spectroscopy ; Ralstonia metallidurans CH34
• 刊名：FEBS Letters
• 出版年：2004
• 出版时间：September 24, 2004
• 年：2004
• 卷：575
• 期：1-3
• 页码：86-90
• 全文大小：147 K

文摘

In MerT, the mercury transporter, a first cysteine pair, located in the first trans-membrane helix, receives mercury from the periplasm. Then, a second cysteine pair, housed in a cytoplasmic loop connecting the second and the third trans-membrane helices, is thought to transfer the metal to another cysteine pair located in the N-terminal extension of the mercuric reductase. We found that a 23-amino acid synthetic peptide corresponding to the cytoplasmic loop can bind one mercury atom per molecule and that this mercury atom can be transferred specifically to MerAa. The solution structure of Hg-bound ppMerT has been solved by ¹H NMR spectroscopy.