Chemical biology studies on norrisolide
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- 作者：Gianni Guizzunti ; Thomas P. Brady ; Derek Fischer ; Vivek Malhotra ; Emmanuel A. Theodorakis
- 关键词：Natural product ; Fluorescent analogues ; Molecular probe ; Scaffolds ; Secretory pathway
- 刊名：Bioorganic and Medicinal Chemistry
- 出版年：2010
- 出版时间：15 March 2010
- 年：2010
- 卷：18
- 期：6
- 页码：2115-2122
- 全文大小：1999 K
文摘
The cellular activity of norrisolide (7), a novel Golgi-vesiculating agent, was dissected as function of its chemical structure. This natural product induces irreversible vesiculation of the Golgi membranes and blocks protein transport at the level of the Golgi. The Golgi localization and fragmentation effects of 7 depend on the presence of the perhydroindane core, while the irreversibility of fragmentation depends on the acetyl group of 7. We show that fluorescent derivatives of norrisolide are able to localize to the Golgi apparatus and represent important tools for the study of the Golgi structure and function.