Carbon nanotube-based antimicrobial biomaterials formed via layer-by-layer assembly with polypeptides

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• 作者：Seyma Aslan ; Marie Deneufchatel ; Sara Hashmi ; Nan Li ; Lisa D. Pfefferle ; Menachem Elimelech ; Emmanuel Pauthe ; Paul R. Van Tassel
• 关键词：Antimicrobial ; Single walled carbon nanotubes ; Biomaterial ; Polyelectrolyte ; Aqueous ; Dispersibility ; Layer-by-layer
• 刊名：Journal of Colloid and Interface Science
• 出版年：2012
• 出版时间：15 December, 2012
• 年：2012
• 卷：388
• 期：1
• 页码：268-273
• 全文大小：910 K

文摘

Biomaterials capable of suppressing microbial infection are of clear importance in various health care applications, e.g. implantable devices. In this study, we investigate the antimicrobial activity of single-walled carbon nanotubes (SWNT) layer-by-layer (LbL) assembled with the polyelectrolytes poly(L-lysine) (PLL) and poly(L-glutamic acid) (PGA). SWNT dispersion in aqueous solution is achieved through the biocompatible nonionic surfactant polyoxyethylene(20) sorbitan monolaurate (Tween 20), and the amphiphilic polymer phospholipid-poly(ethylene glycol) (PL-PEG). Absorbance spectroscopy and transmission electron microscopy (TEM) show SWNT with either Tween 20 or PL-PEG in aqueous solution to be well dispersed, at about the level of SWNT in chloroform. Quartz crystal microgravimetry with dissipation (QCMD) measurements show both SWNT-Tween and SWNT-PL-PEG to LbL assemble with PLL and PGA into multilayer films, with the PL-PEG system yielding the greater final SWNT content. Escherichia coli and Staphylococcus epidermidis inactivation rates are significantly higher (up to 90 % ) upon 24 h incubation with SWNT containing films, compared to control films (ca. 20 % ). This study demonstrates the potential usefulness of SWNT/PLL/PGA thin films as antimicrobial biomaterials.