Generation of a molecular clone of an attenuated lentivirus, a first step in understanding cytopathogenicity and virulence
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- 作者：Laure Blatti-Cardinauxa ; Giuliano Pisonib ; Michael H. Stoffelc ; Reto Zanonia ; Marie-Luise Zahnoa ; Giuseppe Bertonia ; bertoni@besonet.ch" class="auth_mail" title="E-mail the corresponding author
- 关键词：Small ruminant lentiviruses ; Caprine arthritis encephalitis virus ; Visna/maedi virus ; Molecular clone ; Infectious clone ; Electron microscopy ; Long terminal repeats ; Cytopathogenicity ; Transcription ; Virus replication
- 刊名：Virology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：487
- 期：Complete
- 页码：50-58
- 全文大小：1754 K
文摘
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The complete genome of 3 small ruminant lentiviruses field isolates was sequenced.
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Isolate s7385 may be the product of a recombination event between g6221 and s7631.
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Particular LTR mutations may explain the attenuated phenotype of these viruses.
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We synthetized a replication competent molecular clone of isolate g6221.
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Striking ultrastructural differences between cells infected different SRLV.