Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate

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• 作者：Haizhou Lv^a ; Xin Zhang^a ; Baosheng Liao^a ; Wanjing Liu^a ; Liu He^a ; Jingyuan Song^a ; Chao Sun^a ; Hongmei Luo^a ; ^{hmluo@implad.ac.cn" class="auth_mail" title="E-mail the corresponding author} ; Shilin Chen^b ; ^{slchen@implad.ac.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CAS ; cycloartenol synthase ; DXR ; 1-deoxy-d-xylulose-5-phosphate reductoisomerase ; DMAPP ; dimethylallyl diphosphate ; FPS ; farnesyl pyrophosphate synthase ; HDR ; 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase ; HMBPP ; 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate ; IPP ; isopentenyl diphosphate ; MVA ; mevalonic acid ; MEP ; 2-methyl-d-eryth-ritol-4-phosphate ; ORF ; open reading frame ; SQS ; qualene synthase ; SE ; squalene epoxidase
• 刊名：Acta Pharmaceutica Sinica B
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：5
• 期：6
• 页码：583-589
• 全文大小：2268 K

文摘

We cloned and analyzed the two genes of the 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene family from Huperzia serrate. The two transcripts coding HDR, named HsHDR1 and HsHDR2, were discovered in the transcriptome dataset of H. serrate and were cloned by reverse transcription-polymerase chain reaction (RT-PCR). The physicochemical properties, protein domains, protein secondary structure, and 3D structure of the putative HsHDR1 and HsHDR2 proteins were analyzed. The full-length cDNA of the HsHDR1 gene contained 1431 bp encoding a putative protein with 476 amino acids, whereas the HsHDR2 gene contained 1428 bp encoding a putative protein of 475 amino acids. These two proteins contained the conserved domain of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (PF02401), but without the transmembrane region and signal peptide. The most abundant expression of HsHDR1 and HsHDR2 was detected in H. serrate roots, followed by the stems and leaves. Our results provide a foundation for exploring the function of HsHDR1 and HsHDR2 in terpenoid and sterol biosynthesis in Huperziaceae plants.