Expression profiling of the triterpene saponin biosynthesis genes FPS, SS, SE, and DS in the medicinal plant Panax notoginseng

详细信息    查看全文

• 作者：Yunyun Niu ; Hongmei Luo ; Chao Sun ; Tae-Jin Yang ; Linlin Dong ; Linfang Huang ; Shilin Chen
• 关键词：MeJA ; methyl jasmonate ; Ct ; cycle threshold ; ORF ; open reading frame ; EST ; expressed sequence tag ; NCBI ; National Center for Biotechnology Information ; kDa ; kilo Dalton ; pI ; isoelectric point ; MVA ; mevalonic acid ; FPP ; farnesyl diphosphate ; FPS ; farnesyl diphosphate synthase ; SS ; squalene synthase ; SE ; squalene epoxidase ; DS ; dammarenediol-II synthase ; OSCs ; oxidosqualene cyclases ; CYP450 ; cytochrome P450-dependent monooxygenases ; GT ; glycosyltransferases ; NJ ; neighbor-joining ; ML ; maximum likel
• 刊名：Gene
• 出版年：1 January, 2014
• 年：2014
• 卷：533
• 期：1
• 页码：295-303
• 全文大小：1977 K

文摘

Panax notoginseng (Burk) F. H. Chen, an economically significant medicinal plant with hemostatic and health tonic activities, has been used in Traditional Chinese Medicine (TCM) for more than 3000 years. Triterpene saponins are responsible for most of the pharmacological activities of P. notoginseng. Here, we cloned five cDNA sequences encoding the key enzymes involved in triterpene saponin biosynthesis, namely, PnFPS, PnSS, PnSE1, PnSE2, and PnDS, and analyzed the conserved domains and phylogenetics of their corresponding proteins. Their organ-specific expression patterns in four-year-old P. notoginseng were detected by real-time PCR, showing that they were all most highly expressed in flowers. In addition, four of the genes, excluding PnSE2, were upregulated in leaves following stimulation with methyl jasmonate. This study is the first comprehensive analysis of the expression patterns of pivotal genes for triterpene saponin biosynthesis in P. notoginseng and provides a basis to further elucidate the molecular mechanism for the biosynthesis of these medically important compounds.