Mapping of endoglucanases displayed on yeast cell surface using atomic force microscopy

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• 作者：Musashi Takenaka^a ; Takuya Kobayashi^a ; Kentaro Inokuma^b ; Tomohisa Hasunuma^b ; Tatsuo Maruyama^a ; Chiaki Ogino^a ; ^{ochiaki@port.kobe-u.ac.jp} ; Akihiko Kondo^a ; ^b
• 关键词：Atomic force microscope ; Force curve mapping ; Endoglucanase ; Yeast ; Cell surface display
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：151
• 期：Complete
• 页码：134-142
• 全文大小：1793 K
• 卷排序：151

文摘

Two kinds of unique anchoring regions were used for displaying the endoglucanase (EG) on the yeast cell surface. Differences in the display level and the localization were achieved by atomic force microscope (AFM). Interactive force between cellulose and EG was measured via AFM. Force curve mapping revealed the difference in display levels and localization of EG by the anchoring regions. The propose methodology enables to visualize EG on yeast cell surface.