Improving the activity of the endoglucanase, Cel8M from Escherichia coli by error-prone PCR
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- 作者：Ling Lina ; linling8@mail.ahnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Chenggen Fua ; Weiqian Huangb
- 关键词：Endoglucanase ; Error-prone PCR ; Congo red ; 3D-Structural modelling
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：52-58
- 全文大小：2378 K
文摘
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Catalytic efficiency of Cel8M from Escherichia coli was improved by error-prone PCR.
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The variant, Cel8ME15 with substitution of K353E showed 1.42 fold increased activity.
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The substitution of G117S could result in 1.61 fold increased activity in Cel8ME18.
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These results could be applied to improve the activities of GH8 enzymes.