Transferrin receptor expression and role in transendothelial transport of transferrin in cultured brain endothelial monolayers
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- 作者：Maria Hersoma ; Hans Christian Helmsa ; Natasia Pretzera ; Charlotte Goldemana ; Andreas I. Jensenb ; Gregory Severinb ; Morten S. Nielsenc ; René ; Holma ; d ; Birger Brodina ; birger.brodin@sund.ku.dk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Blood-brain barrier ; Receptor-mediated transcytosis ; Transferrin receptor ; Drug delivery ; Cell culture ; Bovine brain endothelial cells ; Rat astrocytes
- 刊名：Molecular and Cellular Neuroscience
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：76
- 期：Complete
- 页码：59-67
- 全文大小：1138 K
文摘
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The transferrin receptor (TfR) has been suggested to ferry drug compounds across the blood-brain barrier.
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TfR expression and function is studied in cultured brain capillary endothelial cells.
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Cultured endothelial cells express the TfR at both the mRNA and protein level.
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Endothelial co-culture with astrocytes induces a tightening of the monolayers, but do not change TfR expression.
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Endothelial monolayers bind transferrin, but transendothelial transferrin transport appears not to be receptor dependent.