Preparation and characterization of electrospun alginate/PLA nanofibers as tissue engineering material by emulsion eletrospinning

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• 作者：Weihong Xu^a ; ¹ ; Renzhe Shen^c ; ¹ ; Yurong Yan^a ; ^{yryan@scut.edu.cn} ; Jie Gao^b ; ^{48014713@qq.com}
• 关键词：Polylatic acid ; Alginate ; Emulsions electrospinning ; Tissue engineering
• 刊名：Journal of the Mechanical Behavior of Biomedical Materials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：65
• 期：Complete
• 页码：428-438
• 全文大小：2772 K
• 卷排序：65

文摘

Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering. Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold. Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility. In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes. The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result. A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA). The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.