Transduction of an immortalized olfactory ensheathing glia cell line with the green fluorescent protein (GFP) gene: Evaluation of its neuroregenerative capacity as a proof of concept
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- 作者：N. Plaza1 ; D. Simó ; n1 ; J. Sierra ; M.T. Moreno-Flores ; mteresa.moreno@uam.es" class="auth_mail" title="E-mail the corresponding author ; mt.moreno.prof@ufv.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：CNS ; central nervous system ; E-GFP ; enhanced green fluorescent protein ; FACS ; fluorescence-activated cell sorting ; FCS ; foetal calf serum ; GFP ; green fluorescent protein ; OEG ; olfactory ensheathing glia ; ihOEG ; immortalized human olfactory ensheathing glia ; LargeT-SV40 ; large T antigen from SV40 virus ; MAP ; microbutule-associated proteins ; PFA ; paraformaldehyde ; PLL ; poly-l-lysine ; RGN ; retinal ganglion neuron ; SEM ; standard error of mean ; TERT ; telomerase catalytic subunit
- 刊名：Neuroscience Letters
- 出版年：2016
- 出版时间：26 January 2016
- 年：2016
- 卷：612
- 期：Complete
- 页码：25-31
- 全文大小：1630 K
文摘
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A constitutively GFP-expressing, immortalized human olfactory ensheathing glia cell line (Ts14-GFP) is generated.
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As a proof of concept, we demonstrate that Ts14-GFP maintains the parental line neuroregenerative properties in in vitro assays.
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A control low-regenerative ihOEG line (Ts12) is also constructed.