Halo-obligatory inteins can be activated only above specific salinity levels.
Salt-induced splicing was used for protein cyclization, purification, and ligation.
Engineered MCM2 intein from H. utahensis has efficient ligation kinetics.
Ser in proteins can be used as the + 1 position for traceless protein ligation.
NMR study of intact TonB protein was demonstrated by segmental isotopic labeling.