Production of pseudorabies virus recombinant glycoprotein B and its use in an agar gel immunodiffusion (AGID) test for detection of antibodies with sensitivity and specificity equal to the virus neutralization assay.
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- 作者：Marí ; a Soledad Serenaa ; b ; Christoph Geislerd ; Germá ; n Ernesto Metza ; b ; Eduardo Carlos Mó ; rtolac ; Marí ; a Gabriela Echeverrí ; aa ; b ; mariagabrielaecheverria@yahoo.com.ar" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pseudorabies ; Aujeszky&rsquo ; s disease ; Agar Gel Immunodiffusion ; Glycoprotein B ; Baculovirus insect cell system
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：230
- 期：Complete
- 页码：9-12
- 全文大小：735 K
文摘
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We used betaine as an enhancer to PCR amplify the 70% GC PrV glycoprotein B ORF.
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We produced a recombinant form of PrV gB in the baculovirus-insect cell system.
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The recombinant gB was used to develop an agar gel immunodiffusion (AGID) test.
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Sensitivity and specificity are comparable to the virus neutralization (VN) assay.
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Our AGID is an affordable, easy to use alternative to the gold standard VN assay.