Dietary interesterified fat enriched with palmitic acid induces atherosclerosis by impairing macrophage cholesterol efflux and eliciting inflammation

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• 作者：Milessa Silva Afonso^a ; ^{milessafonso@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Maria Silvia Ferrari Lavrador^a ; ^{mariasilviaferrari@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} ; Marcia Kiyomi Koike^b ; ^{mkoike2011@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Dennys Esper Cintra^c ; ^{dcintra@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Fabiana Dias Ferreira^a ; ^{fabianaferreira1@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Valeria Sutti Nunes^a ; ^{valeriasutti@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Gabriela Castilho^a ; ^{ga.castilho@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Luiz Antonio Gioielli^d ; ^{lagio@usp.br" class="auth_mail" title="E-mail the corresponding author} ; Renata Paula Bombo^a ; ^{rebombo@terra.com.br" class="auth_mail" title="E-mail the corresponding author} ; Sergio Catanozi^a ; ^{catanozi@usp.br" class="auth_mail" title="E-mail the corresponding author} ; Elia Garcia Caldini^e ; ^{elia@usp.br" class="auth_mail" title="E-mail the corresponding author} ; Nilsa Regina Damaceno-Rodrigues^e ; ^{nilsa@usp.br" class="auth_mail" title="E-mail the corresponding author} ; Marisa Passarelli^a ; ^{mpassere@usp.br" class="auth_mail" title="E-mail the corresponding author} ; Edna Regina Nakandakare^a ; ^{enakonda@usp.br" class="auth_mail" title="E-mail the corresponding author} ; Ana Maria Lottenberg^a ; ^f ; ^{ana.lottenberg@hc.fm.usp.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：PUFA ; Diet enriched with polyunsaturated fatty acids ; TRANS ; Diet enriched with trans fatty acids ; PALM ; Diet enriched with palmitic acid ; PALM INTER ; Diet enriched with palmitic acid in the sn-2 position of triacylglycerols ; STEAR ; Diet enriched with stearic acid ; STEAR INTER ; Diet enriched with stearic acid in the sn-2 position of triacylglycerols ; IL-6 ; Interleukin-6 ; IL-1β ; Interleukin-1β ; IL-10 ; Interleukin-10 ; Ccl2 ; Monocyte chemoattractant protein-1 ; Tnf α ; Tumoral necrosis factor-α ; LPS
• 刊名：Journal of Nutritional Biochemistry
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：32
• 期：Complete
• 页码：91-100
• 全文大小：1510 K

文摘

Interesterified fats are currently being used to replace trans fatty acids. However, their impact on biological pathways involved in the atherosclerosis development was not investigated. Weaning male LDLr-KO mice were fed for 16 weeks on a high-fat diet (40% energy as fat) containing polyunsaturated (PUFA), TRANS, palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR) or stearic interesterified (STEAR INTER). Plasma lipids, lipoprotein profile, arterial lesion area, macrophage infiltration, collagen content and inflammatory response modulation were determined. Macrophage cholesterol efflux and the arterial expression of cholesterol uptake and efflux receptors were also performed. The interesterification process did not alter plasma lipid concentrations. Although PALM INTER did not increase plasma cholesterol concentration as much as TRANS, the cholesterol enrichment in the LDL particle was similar in both groups. Moreover, PALM INTER induced the highest IL-1β, MCP-1 and IL-6 secretion from peritoneal macrophages as compared to others. This inflammatory response elicited by PALM INTER was confirmed in arterial wall, as compared to PALM. These deleterious effects of PALM INTER culminate in higher atherosclerotic lesion, macrophage infiltration and collagen content than PALM, STEAR, STEAR INTER and PUFA. These events can partially be attributed to a macrophage cholesterol accumulation, promoted by apoAI and HDL₂-mediated cholesterol efflux impairment and increased Olr-1 and decreased Abca1 and Nr1h3 expressions in the arterial wall. Interesterified fats containing palmitic acid induce atherosclerosis development by promoting cholesterol accumulation in LDL particles and macrophagic cells, activating the inflammatory process in LDLr-KO mice.