Specific enrichment of a targeted nitrotyrosine-containing peptide from complex matrices and relative quantification for liquid chromatography-mass spectrometry analysis

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• 作者：Yun Yang ^{yangyunwww@126.com yangyunwww@gmail.com}
• 关键词：Nitrotyrosine ; Aminotyrosine ; Enrichment ; Relative quantification ; LC&ndash ; MS
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：17 February 2017
• 年：2017
• 卷：1485
• 期：Complete
• 页码：90-100
• 全文大小：1514 K
• 卷排序：1485

文摘

A novel method to specifically enrich nitrotyrosine-containing peptides from complex biological matrices was developed. Stringency was validated by highly efficient enrichment of nitrated angiotensin II from 1:100 (w/w) BSA digest and from 1:10 000 (w/w) plasma digest. Mean recoveries during enrichment from BSA digest and from plasma digest were 49.8% and 41.1%, respectively. Nitrotyrosine was reduced to aminotyrosine by DTT&hemin to avoid considerable amount of side-products (could be over 50%).