Expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides

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• 作者：&#xc1 ; rpá ; d Csernetics^a ; Gá ; bor Nagy^a ; Enrique A. Iturriaga^b ; Andrá ; s Szekeres^c ; Arturo P. Eslava^b ; Csaba Vá ; gvö ; lgyi^a ; Tamá ; s Papp^a ; ^{pappt@bio.u-szeged.hu"" rel=""nofollow}
• 关键词：Mucor ; β ; -Carotene ; Canthaxanthin ; IPP isomerase ; FPP synthase ; GGPP synthase ; Metabolic engineering
• 刊名：Fungal Genetics and Biology
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：48
• 期：7
• 页码：696-703
• 全文大小：557 K

文摘

The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone.