Ex-Vivo percutaneous absorption of enrofloxacin: Comparison of LMOG organogel vs. pentravan cream

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• 作者：Plamen Kirilov^a ; ^{plamen.kirilov@univ-lyon1.fr" class="auth_mail" title="E-mail the corresponding author} ; Van Hung Tran^a ; Alban Ducrotté ; -Tassel^a ; Jean-Paul Salvi^a ; Sé ; bastien Perrot^b ; Marek Haftek^a ; Roselyne Boulieu^a ; ^c ; Fabrice Pirot^a ; ^d
• 关键词：Enrofloxacin (PubChem CID ; 71188) ; 12-hydroxystearic acid (PubChem CID ; 7789) ; Isopropyl myristate (PubChem CID ; 8042) ; Oleic acid (PubChem CID ; 445639) ; Perchloric acid (PubChem CID ; 24247) ; Phosphate-Buffered Saline (PubChem CID ; 249785514) ; Glycerol (PubChem CID ; 753) ; Polyoxyl 40 stearate (PubChem CID ; 24762) ; Soja lecithin (PubChem CID ; 57369748) ; Simethicone (PubChem CID ; 6433516) ; Demineralized water (PubChem CID ; 962)
• 刊名：International Journal of Pharmaceutics
• 出版年：2016
• 出版时间：10 February 2016
• 年：2016
• 卷：498
• 期：1-2
• 页码：170-177
• 全文大小：640 K

文摘

The objective of this study was to investigate the percutaneous absorption of enrofloxacin from two base formulations, Pentravan^® cream and LMOG organogel. Ex-vivo experiments were carried out on pig ear skin. The percutaneous permeation through pig skin of two formulations containing 5 wt% of enrofloxacin was measured and compared using Franz diffusion cells. At appropriate intervals up to 120 h, diffusion samples were taken and analyzed using HPLC assays. Permeation profiles were established and the parameters T_lag and flux values were calculated. In this ex-vivo study, the flux values were 0.35 μg cm⁻² h⁻¹ for Pentravan^® and 1.22 μg cm⁻² h⁻¹ for LMOG organogel, corresponding respectively to 7.9 % and 29.3 % of enrofloxacin absorbed after 120 h by these formulations. The lag time (T_lag) of Pentravan^® and organogel were 6.32 and 0.015 h respectively. The absorption time to reach the antibiotic concentration of enrofloxacin (2 μg mL⁻¹) in the receptor was 60 h with Pentravan^® and 30 h with the organogel, suggesting more effective treatment by the latter. Enrofloxacin contained in organogel could be absorbed through pig ear skin 3.7 times greater than that in Pentravan^® (commercial formulation). This study demonstrates the perspective of organogel formulations as potential drug delivery systems.