Introducing a cleavable signal peptide enhances the packaging efficiency of lentiviral vectors pseudotyped with Japanese encephalitis virus envelope proteins
The signal peptide gene of VSV G protein was introduced into JEV envelope expression vector. JEV E protein and HIV p24 were present in the same particles of the three pseudotyped JEV-E based lentiviral vectors. Signal peptide enhanced packaging efficiency of pseudotyped JEV-E based lentiviral vector, and a strong signal peptide. Signal peptide does not influence the infectivity of pseudotyped JEV-E based lentiviral vectors.