Comparison of the patterns of antibody recall responses to HIV-1 gp120 and hepatitis B surface antigen in immunized mice

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• 作者：Hao-Tong Yu^a ; ¹ ; Jia-Ye Wang^a ; ^b ; ^c ; ¹ ; Dan Tian^a ; Ming-Xia Wang^a ; Yan Li^a ; ^b ; ^c ; Li Yuan^a ; Wen-Jiang Chen^a ; Di Li^a ; ^b ; Min Zhuang^a ; ^b ; ^c ; ^{zhuangm@ems.hrbmu.edu.cn} ; Hong Ling^a ; ^b ; ^c ; ^d ; ^{lingh@ems.hrbmu.edu.cn}
• 关键词：HIV-1 ; Envelope ; HBsAg ; T follicular helper cells ; PD-1 ; Immune memory
• 刊名：Vaccine
• 出版年：2016
• 出版时间：7 December 2016
• 年：2016
• 卷：34
• 期：50
• 页码：6276-6284
• 全文大小：1609 K
• 卷排序：34

文摘

To date, we still lack an ideal strategy for designing envelope glycoprotein (Env) vaccines to elicit potent protective antibodies against HIV-1 infection. Since the human hepatitis B virus surface antigen (HBsAg) is representative of effective vaccines that can induce ideal humoral immune responses, knowledge of how it elicits antibody responses and T helper cells would be an useful reference for HIV vaccine development. We compared the characteristics of the HIV-1 Env gp120 trimer and HBsAg in antibody elicitation and induction of T follicular helper (Tfh) and memory B cells in immunized Balb/c mice. Using the strategy of protein prime-protein boost, we found that HIV-1 gp120 induced slower recall antibody responses but redundant non-specific IgG responses at early time after boosting compared to HBsAg. The higher frequency of PD-1hiCD4+ T cells and Tfh cells that appeared at the early time point after gp120 boosting is likely to limit the development of memory B cells, memory T cells, and specific antibody recall responses. These findings regarding the different features of HIV envelope and HBsAg in T helper cell responses may provide a direction to improve HIV envelope immunogenicity.