A GH 36 伪-galactosidase from Streptomyces sp. HJG4 showed low-temperature activity.
The Streptomyces 伪-galactosidase (AgaAHJG4) showed alkaline and multiple pH optima.
A GH 36 伪-galactosidase from Mesorhizobium sp. JB07 was lead–zinc tolerant.
The two enzymes showed transglycosylation activity but required different acceptors.
Potential sequence– and structure–function relationships of AgaAHJG4 were presumed.