Metabolic networks to generate pyruvate, PEP and ATP from glycerol in Pseudomonas fluorescens
详细信息 查看全文
- 作者：Azhar Alhasawi ; Sean C. Thomas ; Vasu D. Appanna ; vappanna@laurentian.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：GDH ; glycerol dehydrogenase ; AK ; adenylate kinase ; ACK ; acetate kinase ; CFE ; cell free extract ; G6PDH ; glucose 6-phosphate dehydrogenase ; H2O2 ; hydrogen peroxide ; ICDH ; isocitrate dehydrogenase ; ME ; malic enzyme ; OP ; oxidative phosphorylation ; PC ; pyruvate carboxylase ; PEPC ; phosphoenolpyruvate carboxylase ; PEPS ; phosphoenolpyruvate synthase ; PPDK ; pyruvate orthophosphate dikinase ; ROS ; reactive oxygen species ; NADPH ; reduced nicotinamide adenine dinucleotide phosphate ; TCA ; tricarboxylic acid
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：85
- 期：Complete
- 页码：51-56
- 全文大小：754 K
文摘
- •
Pseudomonas fluorescens can grow in mineral medium with 10% glycerol.
- •
H2O2 triggers a metabolic shift resulting in the enhanced production of ATP, PEP and pyruvate.
- •
The enhanced activities of enzymes like PEPC, PEPS and PC are critical to this process.
- •
Intact cells generate pyruvate while cell-free extract synthesizes ATP and PEP.
- •
This metabolic network may be tailored to the synthesis of these valued-added products from glycerol.