Pseudomonas fluorescens can grow in mineral medium with 10% glycerol.
H2O2 triggers a metabolic shift resulting in the enhanced production of ATP, PEP and pyruvate.
The enhanced activities of enzymes like PEPC, PEPS and PC are critical to this process.
Intact cells generate pyruvate while cell-free extract synthesizes ATP and PEP.
This metabolic network may be tailored to the synthesis of these valued-added products from glycerol.