Evaluation of a lens-free imager to facilitate tuberculosis diagnostics in MODS

详细信息    查看全文

• 作者：Leonardo Solis^a ; Jorge Coronel^b ; Daniel Rueda^a ; Robert H. Gilman^b ; ^c ; Patricia Sheen^a ; ^b ; Mirko Zimic^a ; ^b ; ^{mirko.zimic@upch.pe" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tuberculosis ; MODS ; Multi drug resistance ; MDR ; Diagnostics ; Microscopy ; ePetri ; Lens-free microscopy ; Lens-free imager
• 刊名：Tuberculosis
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：97
• 期：Complete
• 页码：26-32
• 全文大小：2059 K

文摘

Tuberculosis (TB) control efforts are hampered by a mismatch in diagnostic technology. Lack of adequate early diagnostics and Multi-drug resistant (MDR) detection is a critical problem in control efforts. Alternate and novel diagnostic approaches are required, especially in low-resources settings where they are needed most.

The Microscopic Observation Drug Susceptibility (MODS) assay is a cost-effective, highly sensitive, and specific method based on the detection of characteristic cording growth patterns of Mycobacterium tuberculosis (MTB), in microscopic examination of a liquid culture under an inverted microscope. By adding antimicrobials to the wells, MODS also determines antimicrobial susceptibility in both MDR and Extreme Drug Resistant (XDR) tuberculosis.

The interpretation of a MODS culture performed in a 24 well plate, requires an extensive inspection over the entire surface to detect TB cords. This process requires significant time and effort from a trained microscopist.

We evaluated a lens-free imager system, able to render microscopic images of live specimens, for the proof of principle to be used for MODS culture interpretation. The lens-free imager system is able to digitalize a 24-mm² surface with approximately 40X magnification in a single capture.

The evaluation of the lens-free imager found that it produced microscopic images that were adequate for MODS interpretation by a human expert. Compared to the average time that takes a microscopist to completely examine a MODS culture sample, the lens free imager notably reduced the time of inspection.

Therefore, lens-free imager variants may constitute promising systems to aid in the diagnostics of tuberculosis, by simplifying and reducing the time of inspection and permitting automatization of MODS interpretation.