- 作者：Antonia Jelicic Kadic ; Matija Boric ; Marin Vidak ; Lejla Ferhatovic ; Livia Puljak
- 关键词：Diabetes mellitus ; Rat ; Skin ; Nerves ; Maturation
- 刊名：Journal of Tissue Viability
- 出版年：February, 2014
- 年：2014
- 卷：23
- 期：1
- 页码：7-12
- 全文大小：1044 K
Aim
Peripheral nerve fiber depletion in patients with chronic diabetes mellitus (DM) was linked to neuropathic symptoms, development of pain, foot ulcerations and lower extremity amputation. The aim of this study was to analyze cutaneous changes, including paw epidermal thickness and intraepidermal nerve fiber (IENF) density in long-term diabetes, in rats 6 months and 12 months after induction of diabetes.Materials and methods
Epidermal thickness and IENF density were studied in Sprague-Dawley diabetic rats 6 months and 12 months after diabetes induction with streptozotocin. Epidermal thickness was evaluated using hematoxylin and eosin staining. Peripheral nerve fibers were stained with polyclonal antiserum against protein gene product 9.5 (PGP 9.5). Successful diabetes induction was validated by measuring plasma glucose and body mass regularly throughout the experiment.
Results
This study showed that long-term diabetes, induced in Sprague-Dawley rats with streptozotocin, is characterized with significant epidermal thinning and reduction of intraepidermal nerve fibers, 6 months and 12 months after induction of diabetes.
Conclusion
Long-term studies of streptozotocin models of diabetes could be used for making normative IENF densities that can be later used as age-dependent normative values for studying new treatment modalities.