Skin integrated with perfusable vascular channels on a chip

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• 作者：Nobuhito Mori^a ; Yuya Morimoto^a ; Shoji Takeuchi^a ; ^b ; ^{takeuchi@iis.u-tokyo.ac.jp}
• 关键词：Skin-equivalent ; Skin model ; Vascular channel ; Perfusion ; Blood vessel ; Percutaneous absorption
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：116
• 期：Complete
• 页码：48-56
• 全文大小：3240 K
• 卷排序：116

文摘

This paper describes a method for fabricating perfusable vascular channels coated with endothelial cells within a cultured skin-equivalent by fixing it to a culture device connected to an external pump and tubes. A histological analysis showed that vascular channels were constructed in the skin-equivalent, which showed a conventional dermal/epidermal morphology, and the endothelial cells formed tight junctions on the vascular channel wall. The barrier function of the skin-equivalent was also confirmed. Cell distribution analysis indicated that the vascular channels supplied nutrition to the skin-equivalent. Moreover, the feasibility of a skin-equivalent containing vascular channels as a model for studying vascular absorption was demonstrated by measuring test molecule permeation from the epidermal layer into the vascular channels. The results suggested that this skin-equivalent can be used for skin-on-a-chip applications including drug development, cosmetics testing, and studying skin biology.