Molecular analysis of RNA1 and RNA2 sequences from a betanodavirus isolated from giant grouper (Epinephelus lanceolatus) in Australia

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• 作者：Kalpana Agnihotri^a ; ^{Kalpana.Agnihotri@daf.qld.gov.au} ; Brad Pease^a ; ^b ; Roger Chong^a
• 关键词：Betanodavirus ; Giant grouper ; Australia
• 刊名：Virology Reports
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：Complete
• 页码：25-31
• 全文大小：695 K
• 卷排序：6

文摘

Australian aquaculture industry relies on their Asian counterparts for reports on grouper diseases. The diagnosis for betanodavirus infections is based on histopathology and use of RT-qPCR.Isolation of virus is recommended by OIE, and is not pursued for routine diagnostic purposes. The RNA1 and RNA2 segments were sequenced from isolated virus and nucleotide sequences were compared with GenBank sequences. Both these sequences clustered within RGNNV genotype and showed high sequence identity to sequences affecting other grouper species.