miR-145 modulates lncRNA-ROR and Sox2 expression to maintain human amniotic epithelial stem cell pluripotency and β islet-like cell differentiation efficiency
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- 作者：Gang Zoua ; Te Liua ; b ; te.liu@yale.edu" class="auth_mail" title="E-mail the corresponding author ; Lihe Guoc ; Yongyi Huangd ; Ya Fenge ; Qin Huangc ; Tao Duana ; tduan@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：lncRNA-ROR ; long noncoding RNA ROR ; HuAECs ; human amniotic epithelial stem cells ; miR-145 ; microRNA-145 ; T1D ; type 1 diabetes ; ESCs ; embryonic stem cells ; iPSCs ; induced pluripotent stem cells ; TF ; transcription factor ; DMEM ; Dulbecco's modified Eagle's medium ; qRT-PCR ; quantitative real-time PCR ; FISH ; RNA Fluorescence In situ hybridization ; TBST ; Tris-buffered saline containing Tween 20
- 刊名：Gene
- 出版年：2016
- 出版时间：10 October 2016
- 年：2016
- 卷：591
- 期：1
- 页码：48-57
- 全文大小：1307 K
文摘
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miR-145 silences lncRNA-ROR and Sox2 expression via binding of specific sites on them.
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Inhibiting lncRNA-ROR weakens the efficiency of HuAECs differentiated into β islet cells.
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lncRNA-ROR effectively maintains Sox2 expression via competitive binding to miR-145.