Synthetic strategies for efficient conjugation of organometallic complexes with pendant protein reactive markers

详细信息    查看全文

• 作者：Dominik Jantke ; Alex ; er N. Marziale ; Thomas Reiner ; Florian Kraus ; Eberhardt Herdtweck ; Andreas Raba ; J?rg Eppinger
• 关键词：Bioinorganic chemistry ; Metalloenzymes ; Peptide coupling ; Protease inhibitors
• 刊名：Journal of Organometallic Chemistry
• 出版年：2013
• 出版时间：1 November, 2013
• 年：2013
• 卷：744
• 期：Complete
• 页码：82-91
• 全文大小：1004 K

文摘

Site-directed conjugation of metal centers to proteins is fundamental for biological and bioinorganic applications of transition metals. However, methods for the site-selective introduction of metal centers remain scarce. Herein, we present broadly applicable synthetic strategies for the conjugation of bioactive molecules with a range of organometallic complexes. Following three different synthetic strategies, we were able to synthesize a small library of metal conjugated protein markers featuring different types of protein reactive sites (epoxides, phenylphosphonates, fluorosulfonates and fluorophosphonate groups) as well as different late transition metals (iron, ruthenium, rhodium, palladium and platinum). The products were isolated in moderate to excellent yields and high purity. Furthermore, X-ray diffraction of the metalated protein markers corroborates structural integrity of the metal complex and the protein reactive site.