DNase H Activity of Neisseria meningitidis Cas9
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- 作者：Yan Zhang1 ; 4 ; Rakhi Rajan2 ; 4 ; 5 ; H. Steven Seifert3 ; Alfonso Mondragó ; n2 ; Erik J. Sontheimer1 ; erik.sontheimer@umassmed.edu" class="auth_mail" title="E-mail the corresponding author
- 刊名：Molecular Cell
- 出版年：2015
- 出版时间：15 October 2015
- 年：2015
- 卷：60
- 期：2
- 页码：242-255
- 全文大小：3615 K
文摘
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NmeCas9 is a dual RNA-guided dsDNA endonuclease like other Cas9 orthologs
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NmeCas9 efficiently cleaves ssDNA in an RNA-guided, tracrRNA-independent manner
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NmeCas9 “DNase H” cleavage requires no specific sequence in the RNA guide
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NmeCas9’s “DNase H” cut sites are measured from the 5′ end of the RNA-paired region