Determination of α-hydroxy acids in cosmetic products by capillary electrophoresis

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• 作者：Elizâ ; ngela Abreu Dutra ; Maria Inê ; s Rocha Miritello Santoro ; Gustavo Amadeu Micke ; Marina Franco Maggi Tavares and Erika Rosa Maria Kedor-Hackmann
• 关键词：α ; -Hydroxy acids ; Cosmetics ; Capillary electrophoresis ; Indirect detection ; Validation
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2006
• 出版时间：13 February 2006
• 年：2006
• 卷：40
• 期：2
• 页码：242-248
• 全文大小：128 K

文摘

In this work, a simple and reliable method for the simultaneous analysis of α-hydroxy acids such as tartaric, glycolic and lactic acids in cosmetic products was developed using capillary electrophoresis with indirect UV detection at 254 nm. A buffer solution containing 10 mmol l⁻¹ potassium phthalate (pH 4.1) and 0.5 mmol l⁻¹ cetyltrimethylammonium bromide as electroosmotic flow modifier allowed baseline resolution of the analytes in approximately 3 min. A few validation parameters of the proposed method include: good linearity for all compounds in the range from 10 to 100 mg l⁻¹ with coefficients of correlation larger than 0.9999. The average recoveries of tartaric, glycolic and lactic acids in commercial samples were 99.12, 99.41 and 99.43 % , respectively. The method presented acceptable precision with average relative standard deviation of 0.54 % (assay of commercial samples), 0.44 % (peak area) and 0.16 % (migration time).