Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region
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- 作者：Pergolizzi ; Rossana ; Appierto ; Valentina ; Bosetti ; Alessandro ; DeBellis ; GianLuca ; Rovida ; Ermanna ; et. al.
- 关键词：Human X chromosome ; automated DNA sequencing ; homology search
- 刊名：Gene
- 出版年：1996
- 出版时间：February 12, 1996
- 年：1996
- 卷：168
- 期：2
- 页码：267-270
- 全文大小：456 K
文摘
To contribute to the isolation of genes within the q24-qter region of the human X chromosome, we screened three cDNA libraries (human fetal brain, liver and skeletal muscle) with a cosmid clone containing a CpG island previously mapped in the q28 region. A full-length 2.1-kb cDNA clone was isolated (XIB); DNA databank searches revealed identity with an EST fragment (XAP-1), residing between the RCP/GCP and G6PD loci. The XIB coding region (909 bp) showed 44 % amino acid (aa) identity to pig DNase I. Several conserved residues have been observed between these two genes including aa in the active site. XIB expressed a single transcript in adult heart and skeletal muscle, whereas, in some fetal tissues, two different-sized transcripts were seen. Zoo blot analysis showed a remarkable cross-species conservation. Expression and sequence of this novel gene are reported.