Characterisation of esterolytic activity from two wild mushroom species, Amanita vaginata
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- 作者：Nagihan Saglam Ertunga ; Ü ; mmü ; han Cakmak ; Ahmet Colak ; Ö ; zlem Faiz ; Ertugrul Sesli
- 关键词：Esterase ; Amanita vaginata var. vaginata ; Tricholoma terreum ; Mushroom
- 刊名：Food Chemistry
- 出版年：2009
- 出版时间：15 August 2009
- 年：2009
- 卷：115
- 期：4
- 页码：1486-1490
- 全文大小：266 K
文摘
Characterisation of esterase activities from the edible mushroom species, Amanita vaginata var. vaginata and Tricholoma terreum, were investigated. Native electrophoresis of the crude extracts prepared from both mushroom samples showed the presence of esterolytic activities. The extracts had the greatest activity in the presence of p-nitrophenyl butyrate (pNPB) as a substrate. pH and temperature optima were found to be 8.0 and 30 °C for both enzymes, respectively. Vmax and Km values were determined as 14.2 U/l and 71 μM for A. vaginata var. vaginata and 34.6 U/l and 9.6 μM for T. terreum, respectively. The pH-stability profile showed a stationary line between 3.0 and 10.0 for both enzymes. The esterolytic activities from the extracts were maintained between 10 and 40 °C for 4 h and started to decrease at 50 °C. The effects of EDTA, NaN3, DTT and PMSF on the enzyme activity were also investigated.