Effect of honokiol on erythrocytes

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• 作者：Mohanad Zbidah ; Adrian Lupescu ; Tabea Herrmann ; Wenting Yang ; Michael Foller ; Kashif Jilani ; Florian Lang
• 关键词：Phosphatidylserine ; Honokiol ; Calcium ; Cell volume ; Eryptosis
• 刊名：Toxicology in Vitro
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：27
• 期：6
• 页码：1737-1745
• 全文大小：719 K

文摘

Honokiol ((3,5-di-(2-propenyl)-1,1-biphenyl-2,2-diol), a component of Magnolia officinalis, stimulates apoptosis and is thus considered for the treatment of malignancy. In analogy to apoptosis of nucleated cells, erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and by breakdown of cell membrane phosphatidylserine asymmetry with phosphatidylserine-exposure at the erythrocyte surface. Eryptosis may be triggered following increase of cytosolic Ca²⁺-activity ([Ca²⁺]_i). The present study explored, whether honokiol elicits eryptosis. Cell volume has been estimated from forward scatter, phosphatidylserine-exposure from annexin V binding, hemolysis from hemoglobin release, [Ca²⁺]_i from Fluo3-fluorescence, and ceramide from fluorescent antibodies. As a result, a 48 h exposure to honokiol was followed by a slight but significant increase of [Ca²⁺]_i (15 ¦ÌM), significant decrease of forward scatter (5 ¦ÌM), significant increase of annexin-V-binding (5 ¦ÌM) and significant increase of ceramide formation (15 ¦ÌM). Honokiol further induced slight, but significant hemolysis. Honokiol (15 ¦ÌM) induced annexin-V-binding was significantly blunted but not abrogated in the nominal absence of extracellular Ca²⁺. In conclusion, honokiol triggers suicidal erythrocyte death or eryptosis, an effect at least in part due to stimulation of Ca²⁺ entry and ceramide formation.