Jak-Stat signaling pathway may play a role in the pathogenesis of cholesteatoma

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• 作者：Gö ; rkem Eskiizmir ; MD^a ; ^{gorkemeskiizmir@yahoo.com" class="auth_mail} ; H. Seda Vatansever ; MD^b ; Erdogan Ö ; zgü ; r ; MD^a ; As谋m Aslan ; MD^a ; Gö ; kç ; e Tanyeri ; MD^a ; Derya Gö ; zü ; aç ; 谋k ; PhD^a ; M. Kemal Ö ; zbilgin ; MD^b ; Cemal Cingi ; MD^c
• 刊名：American Journal of Otolaryngology
• 出版年：March-April, 2014
• 年：2014
• 卷：35
• 期：2
• 页码：130-136
• 全文大小：3031 K

文摘

Purpose

Jak-Stat signaling pathway is one of the major signal transduction cascades which regulates most of the cellular events such as cell proliferation, differentiation, cell migration and apoptosis. This study aims to determine the activity of Jak-Stat signaling pathway in the pathogenesis of cholesteatoma.

Materials and Methods

Cholesteatoma and skin samples were obtained from 10 patients who underwent tympanomastoidectomy for chronic otitis media with cholesteatoma. Immunohistochemical analysis of cholesteatoma and skin was performed using anti-Jak1, anti-Jak2, anti-Jak3, anti-Stat1, anti-Stat2, anti-Stat3, anti-Stat4 and anti-Stat5 antibodies. The immunoreactivities in cholesteatoma and skin were quantified using H-score measurement and statistical comparison was performed.

Results

Jak1, Jak2, Jak3, Stat1 and Stat3 immunoreactivities were not detected in cholesteatoma; in contrast to the skin (129.8; 226.7; 33.0; 66.4;115.9). In addition, when H-score measurements of Stat2, Stat4 and Stat5 immunoreactivities were compared between cholesteatoma (172.8; 166.7; 120.0) and skin (400.0; 284.9; 292.0), statistically significant differences were found (p < 0.0001, p < 0.0001, p < 0.0001).

Conclusions

A remarkable deficiency in the family members of Jak-Stat signaling pathway was demonstrated in cholesteatoma. Therefore, perturbations in Jak-Stat signaling pathway may play a role in the pathogenesis of cholesteatoma.