Complete genome characterization of enterovirus 104 circulating in Northern Italy shows recombinant origin of the P3 region

详细信息    查看全文

• 作者：Antonio Piralla ; Loretta Fiorina ; Cristina Daleno ; Susanna Esposito ; Fausto Baldanti
• 关键词：Respiratory infection ; EV-C104 ; Complete genome ; Phylogenetic analysis ; Recombination
• 刊名：Infection, Genetics and Evolution
• 出版年：December, 2013
• 年：2013
• 卷：20
• 期：Complete
• 页码：111-117
• 全文大小：3340 K

文摘

Human enterovirus 104 (EV-C104) is a member of the Human Enterovirus species C (Family Picornaviridae, Genus Enterovirus) and has been associated with mild respiratory syndromes. At present, only two EV-C104 complete genome sequences from strains detected in Switzerland and Japan have been deposited in GenBank. In this study a complete genome analysis of seven Italian EV-C104 strains was carried out. In addition, VP1 sequence analysis was performed in an additional 5 Italian strains (for a total of 12 strains). The genome length of the seven strains was 7406 nucleotides (nt). The seven genomes showed 91.0-96.9% nucleotide identity with respect to other available EV-C104 complete genomes. The P1 and P2 regions of the Italian strains were closely related to EV-C104 identified in Switzerland, while the P3 region was closely related to the EV-C117 strain. In addition, bootscan analysis showed the presence of one putative recombination breakpoint between the P2 and P3 regions. Based on the trees constructed with partial VP1/2A nucleotide sequences, as well as the 3D partial coding region tree, the Italian strains appear to form a single and independent cluster together with the EV-C104 Japanese strain. In conclusion, a complete phylogenetic analysis of the relationship between EV-C104 and other known HEV-C strains was achieved. In addition, the recombinant origin of EV-C104, which has circulated in Italy and Japan, was demonstrated.