The EB66® cell line as a valuable cell substrate for MVA-based vaccines production
详细信息 查看全文
- 作者：Arnaud Lé ; ona ; arnaud.leon@valneva.com" class="auth_mail" title="E-mail the corresponding author ; Anne-Laure Davida ; Brice Madelinea ; Laurence Guianvarc&rsquo ; ha ; 1 ; Elodie Dureaua ; Patrick Champion-Arnauda ; 2 ; Matthias Hebbena ; 1 ; Thierry Hussb ; Benoî ; t Chatrenetc ; Klaus Schwamborna
- 关键词：CEF ; chicken embryo fibroblast ; CEV ; cell-associated enveloped virus ; DMEM ; Dulbecco&rsquo ; s Modified Eagle&rsquo ; s Medium ; DNA ; deoxyribonucleic acid ; ELISA ; enzyme-linked immunosorbent assay ; EMEM ; Eagle&rsquo ; s minimum essential medium ; FBS ; Foetal Bovine Serum ; GFP ; green fluorescent protein ; GMP ; Good Manufacturing Practice ; HCD ; host cell DNA ; HCP ; host cell protein ; HCV ; hepatitis C virus ; HDF ; Human dermal fibroblast ; IGBMC ; Institut de Gé ; né ; tique et de Biologie Molé ; c
- 刊名：Vaccine
- 出版年：2016
- 出版时间：21 November 2016
- 年：2016
- 卷：34
- 期：48
- 页码：5878-5885
- 全文大小：1791 K
文摘
- •
EB66® cell line is a valuable cell substrate for production of MVA-based vaccine.
- •
Pilot-scale production process was established that yields high titres.
- •
Pilot-scale purification process has been developed.
- •
Virus attenuation and transgene expression are maintained in EB66® cells.