Biphasic influence of PGE2 on the resorption activity of osteoclast-like cells derived from human peripheral blood monocytes and mouse RAW264.7 cells
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- 作者：Anne-Helen Luttera ; b ; lutter@b-tu.de" class="auth_mail" title="E-mail the corresponding author ; Ute Hempela ; Ursula Andererb ; Peter Dietera
- 关键词：αMEM ; Alpha Minimum Essential Medium ; cAMP ; cyclic adenosine monophosphate ; DMEM ; Dulbecco's Modified Eagle's Medium ; EDTA ; ethylenediaminetetraacetic acid ; ERK ; extracellular signal-regulated kinases ; FBS ; fetal bovine serum ; GPCR ; G-protein-coupled receptor ; hPBMC ; human peripheral blood mononuclear cell ; MCSF ; macrophage colony-stimulating factor ; OC ; osteoclast ; ODEM ; osteoblast-derived extracellular matrix ; PBS ; phosphate buffered saline ; PGE2 ; prostaglandin E2 ; PI3K ; posphatidylinositol-3
- 刊名：Prostaglandins, Leukotrienes and Essential Fatty Acids
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：111
- 期：Complete
- 页码：1-7
- 全文大小：6739 K
文摘
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PGE2 is an important pro-inflammatory mediator regulating osteoclast function.
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The effect of PGE2 on TRAP and resorptive activity of osteoclastic cells was studied.
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PGE2 inhibited the differentiation of human and mouse osteoclast precursor cells.
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PGE2 had no influence on already differentiated human and mouse osteoclasts.
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Downregulation of PGE2 receptors enables osteoclasts function during inflammation.